Holden opened the penultimate day of Olympic competition with a silver medal in the women's time trial. She covered the 18-mile course in 42 minutes flat.
The five-time national champion from Colorado Springs, Colo., bounced back after a rough trip in the Olympic road race earlier in the week. Mechanical problems and a crash knocked her out of the medal chase there.
Holden finished second to Leontien Zijlaard of the Netherlands, who won her third gold medal of the Sydney Games. Jeannie Longo-Ciprelli of France, soon to turn 42, won the bronze.
It was the second cycling medal for the United States, coming after Marty Nothstein's gold in match sprint.
After the first events Saturday, the Americans remained atop the medal table with 80 (33 gold, 20 silver, 27 bronze). Russia was next with 61 (20-19-22), followed by China's 56 (26-15-15).